e el h6156  (Elabscience Biotechnology)

Journal: bioRxiv

Article Title: Low-intensity vibration (LIV) cellular mechanotherapy reprograms tumor transcriptomes to suppress cancer-promoting inflammation

doi: 10.64898/2026.04.07.716739

Figure Lengend Snippet: (A) Schematic of the custom-built vibration system, which uses a rigidly mounted gun with adjustable stoppers for tuning vibration amplitude to ensure reproducible mechanical stimulation. (B) Overview of mechanotransduction: extrinsic mechanical forces are converted into biochemical signals that activate downstream pathways, leading to changes in gene expression, protein synthesis, and cell phenotype—thereby, regulating cellular proliferation, differentiation, and apoptosis. (C) Calibration of vibration frequencies at different gear settings: gears 1, 2, and 4 corresponded to 90 Hz, 150 Hz, and 300 Hz, respectively, measured at the plate bottom. Vibration frequency varied inversely with displacement amplitude ( n = 4; p < 0.05). (D) Supernatant analysis of LPS-stimulated PBMCs showed a linear relationship between LPS concentration (0– 10 µg/ml) and IL-6 secretion ( p < 0.05). Maximal PBMC proliferation was observed at 5 μg/ml of LPS ( n = 3). (E) Fold change (FC) of IL-6 cytokine secreted by vibrated PBMCs showed most reduction at the 90 Hz LIV ( n = 3). (F) Time-course of IL-6 secretion from LPS-stimulated PBMCs revealed peak cytokine levels at 48 h ( n = 3). (G) LIV increases the supernatant pH of LPS-stimulated PBMCs in a frequency-dependent manner ( n = 4). (H) LIV did not significantly alter the proliferation of MDA-MB-231 cells compared to static controls ( n = 5; p = 0.374).

Article Snippet: The kits utilized included the human IL-6 ELISA Kit from Elabscience (E-EL-H6156), the IFN-γ ELISA kit from Invitrogen (HHC4021), the CXCL12β ELISA Kit from Invitrogen (EHCXCL12B), the MCP-1 ELISA Kit (BMS281INST), the TNFα ELISA Kit from Invitrogen (KHC3014), and the IL-10 ELISA Kit from Elabscience (EHIL10).

Techniques: Gene Expression, Concentration Assay

Journal: bioRxiv

Article Title: Low-intensity vibration (LIV) cellular mechanotherapy reprograms tumor transcriptomes to suppress cancer-promoting inflammation

doi: 10.64898/2026.04.07.716739

Figure Lengend Snippet:

Article Snippet: The kits utilized included the human IL-6 ELISA Kit from Elabscience (E-EL-H6156), the IFN-γ ELISA kit from Invitrogen (HHC4021), the CXCL12β ELISA Kit from Invitrogen (EHCXCL12B), the MCP-1 ELISA Kit (BMS281INST), the TNFα ELISA Kit from Invitrogen (KHC3014), and the IL-10 ELISA Kit from Elabscience (EHIL10).

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: bioRxiv

Article Title: Low-intensity vibration (LIV) cellular mechanotherapy reprograms tumor transcriptomes to suppress cancer-promoting inflammation

doi: 10.64898/2026.04.07.716739

Figure Lengend Snippet: Cytokine concentrations in cell culture supernatants were quantified by ELISA (mean ± SD) and statistical significance was determined by Student’s t-test. (A) IL-6 concentration in vibrated PBMCs was significantly reduced compared to static control (488.26±31.49 pg/ml vs. 573.76± 38.66pg/ml, p = 0.001, n = 7). (B) IFNγ in LIV-treated cells was below the lower limit of detection (LOD) compared to static control concentration of 854.41± 36.01 pg/ml, p < 0.001, n = 5). (C) IL-10 concentration in vibrated PBMCs showed a non-significant increase over control (2.8± 0.83 pg/ml vs. 2.0± 0.70 pg/ml, + 40 %; p = 0.08, n = 5). (D) MCP-1 concentration increased markedly in vibrated PBMCs (4305± 975 pg/ml vs. 2001± 1183 pg/ml, + 115 %; p = 0.02, n = 4). (E) IL-6 in vibrated MDA-MB-231 cells was significantly lower than in controls (109.93± 9.03 pg/ml vs. 171.35± 39.61 pg/ml, − 36 %, p = 0.02, n = 4). (F) TNF-α levels did not differ significantly between vibrated and control MDA-MB-231 cells (24.57± 3.22 pg/ml vs. 28.88± 3.17 pg/ml, − 15 %; p = 0.17, n = 3). (G) CXCL-12β in LIV-treated MDA-MB-231 cells was below the LOD, whereas controls measured at 56.25 ± 39.40 pg/ml ( p = 0.03, n = 4). (H) In co-culture models, IL-6 levels were higher in 2D than in 3D spheroids under LIV (6518± 501 pg/ml vs. 5351± 355 pg/ml, − 22%; p = 0.001, n = 8). (I) IL-6 concentration in vibrated 3D co-cultures was significantly reduced compared with static controls (5351± 355 pg/ml vs. 7104± 392 pg/ml, − 25 %, p < 0.001, n = 8). (J) IL-6 levels in 2D co-cultures did not differ significantly between vibrated and control groups (6518± 501 pg/ml vs. 6887± 483 pg/ml, −5%; p = 0.16, n = 8).

Article Snippet: The kits utilized included the human IL-6 ELISA Kit from Elabscience (E-EL-H6156), the IFN-γ ELISA kit from Invitrogen (HHC4021), the CXCL12β ELISA Kit from Invitrogen (EHCXCL12B), the MCP-1 ELISA Kit (BMS281INST), the TNFα ELISA Kit from Invitrogen (KHC3014), and the IL-10 ELISA Kit from Elabscience (EHIL10).

Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, Concentration Assay, Control, Co-Culture Assay